Dr. S.I Shelley: My work was submitted at the UNU–IRRI workshop on nitrogen fixation in the rhizosphere of rice. Work was valued and brings economic welfare developing countries. Using my methods using anaerobic bacteria to fix atmospheric nitrogen. There were 15 proposal my proposal was top of the list; it brings lot of significance in all projects. There was my name is the top of lists see ongoing book and edited by me as editor to attended that works among all soviet scientist, I was selected to join that workshop.
There were distinguished 15 scientists. Different countries of the world I was one of them. please see binding book.
USSR academy of sciences, institute microbiology and Moscow Timitr Yazev academy. Moscow USSR.
2. Dr. S. K. Lee institute of agricultural science, ministry of agriculture and fisheries Suwon Korea.
3. Dr. Wang. Institute O Botany, Chinese academy of sciences, Beijing, china
4. Dr. J.R Milam of microbiology and cell science, university of Florida, Gainesville USA.
5. Dr. Jarya Boonjawat, department of biochemistry Chulalongkorn university Bangkok Thailand
6. Dr. A. Adelayoaculty of Agriculture University of life university Lieile Nigeria.
7. Dr. A.P Ruschel Centro de Energies nuclear agriculture Cena sao Paula Brazil.
8. Dr. Y Doumergue’s laboratories de microbiologic des sols.
9. Dr. S. K. Kulasooriya dept of botany university of Peradeniya, Peradeniya Sri Lanka
10. Dr. I. Watanabe soil microbiology dept IRRI college laguna Philippines’
11. Dr. M. Umali Garcia College of forestry UPLB college laguns Philippines,
12. Dr. Balandreau. Center DE pedologic biology due CNRS. Van oeuvre Cedex France
13. Dr. Z Shamsuddin dept of soil science university of Pertain Selangor Malaysia and
14. Dr. K. An Ahmed genetics department F faculty of agriculture Cairo university Egypt.
15. Dr. W. T Tucker from research school or biological sciences Australian national university, Canberra, Australia.
Note: all above mentioned distinguished scientist accept my proposal and praised my workout: anaerobic nitrogen fixation and soil fertility.
Explanation’s discussions and conclusions.
I worked along with above mentioned scientist. All were distinguished .it was so nice and pleasure to work with them in these mentioned field and developed new technology biological nitrogen fixation and developed biological nitrogen fixations method and brings lot of significance in agricultural biotechnology.
1. The United Nations university is organizing a workshop on nitrogen fixation in the rhizosphere rice which will be co-sponsored and hosted by the international rice research institute RRI is Lisboans Philippines’ 28th April 4th May 1984. The overall aim was of this workshop is to establish a network of institution cooperating in this field of research.
2. The purpose letter invitation to invite to participate in the workshop Dr Robert Kokke senior program officer development division .letter says in the scope of its commitment to strengthen the biotechnology research capacity of institutions in developing countries , as a whole develop economic in the world through their search , the un university is working to establish a co-ordinate research projects on nitrogen fixation in the rhizosphere of rice still that work going on and implemented IRRI and brings lot of economic welfare to the developing country..
3. As know it is well known that rice is the stable food of the fifths of the world populations and that high nitrogen inputs are required for good yields part or possible all this nitrogen requirements may eventually be met through biological fixation from the atmosphere. Researchers at the Japanese national institute of genetics under the leadership of Dr. Y. Ukionori Hrotha have found some rice strains which have a capacity to use nitrogen fixed by microorganisms and Dr. Shelley found anaerobes are fixing nitrogen. They are now attempting to transfer capacity by in the rhizosphere where oxygen not available, so anaerobes are important said Dr. Shelley. microorganisms high yielding cultivars and former Ussr Dr. Shelley and processor dr. Of sciences V. T. Emtsev found anaerobic genus clostridium to fix atmospheric nitrogen please see his articles with professor Emtsev. And thus Dr. Shelley was invited in same year to attended crop response correlation studies and his research work anaerobic nitrogen fixing bacteria genus clostridium was very much acceptable and his suggestions was accepted publish in newspaper Bangladesh observer and paper was published in book in conference proceedings too in first page’s crop response correlation studies please see attached documents. The approach is multi-disciplinary involving molecular biologics. Plant breeder and soil scientist and others too. The results of this research are more readily available since it is carried out primarily in institutions rather than industrial laboratories by catalyzing a greater interest in this field in developing countries the un universities hopes to create core groups of researchers which will then have the capacity to deal with other topics in the field of biotechnology.
4. 4. Is happy to say or important to say the UN university has chosen to work on biological nitrogen fixation for several reasons. The research will be immediate importance to developing countries and was implemented. And benefited. The results of this research are more readily available since it is carried out primarily in institutions rather than industrial laboratories by catalyzing a greater interest in this field in developing countries the un universities hopes to create core groups of researchers which will then have the capacity to deal with other topics in the field of agricultural biotechnology.
5. A modest amount of the university funding has been allocated to this project for research.
Now about agricultural biotechnology:
I submitted at the unit–irri workshop on nitrogen fixation in the rhizosphere of rice.
There were, 15 proposal my proposal was top of the list, it brings lot of significance in the al the projects. There was my name is the top of the list please see the book and edited by me, to attend that works among all soviet scientist I was selected to join that workshop
there were dr. W. T. Tucker research school of biological sciences, Australian national university Canberra, Australia. Dr. S. k. lee institute of agricultural science, ministry of agriculture and fisheries Suweon Korea.
institute of botany Chinese academy of sciences Beijing, China.
Dr. J. R. Milam dept of microbiology and cell science university of Florida Gainesville. USA.
Dr. Jarya Boonjawat dept of biochemistry Chulalongkorn university Bangkok, Thailand.
Dr. A. Adelayo faculty of agriculture university of life in Nigeria.
Dr. A. P Ruschel Centro de energia nuclear na agriculture cena sao Paula brazil. Dr y. Dommergues laboratories de microbiologic des sols. O. R.s.t. o m Dakar Senegal
Dr. S. A Kulasooriya dept of botany university of Peradeniya in Sri Lanka.
Dr. I Watanabe soil microbiology dept irri college laguna Philippines
Dr. M. Umali Garcia college of forestry uplb college laguna Philippines.
Dr. J. Balandreau
Centre de pedologic biologize due cnrs. Vandoeuvre Cedex France.
Dr. Z Shamsuddin dept of soil science university of Pertania Selangor Malaysia. And Dr. K. An Ahmed genetics department faculty of agriculture, Cairo university Egypt.
I worked among with above mentioned scientist all were distinguished scientist it was pleasure to work with them in thecae field e together developed biological nitrogen fixation and it brings lot of significances in agricultural biotechnology.
1. The United Nations university is organizing a workshop on nitrogen fixation in the rhizosphere of rice which will be co –sponsored and hosted by the international rice research institute is banes, Philippines 28 April -4 May 1984. The overall aim of this workshop is to establish a neork of institutions cooperating in this file of research
2. The purpose of this letter invitation to invite me Dr. Shelley to participate in the workshop. First. Let me Dr. Robert kokke senior program officer development division .in the scope of its commitment to strengthen the biotechnology research capacity of institutions in developing countries, the un university is working to establish a co-ordinate research projects on nitrogen fixation in the rhizosphere of rice still that work going on and implemented IRRI and brings lot of economic welfare to the developing country.
3. As know it is well –known that rice is the stable food of three –fifths of the world populations and that high nitrogen inputs are required for good yields. Part or possible this entire nitrogen requirement may eventually be met through biological fixation of nitrogen from the atmosphere. Researchers at the Japanese national institute of genetics under the leadership of Dr. Y. U Hirota have found some rice strains which have a capacity to use nitrogen fixed by microorganisms living in the rhizosphere. They are now attempting to transfer this capacity too high –yodeling cultivars. And former Ussr Dr. S. I. Shelley and professor Dr. of sciences v.t Emtsev found anaerobic bacteria genus clostridium to fix atmospheric nitrogen see his articles and dr. Shelley was invited in same year to attended to Bangladesh crop response correlations studies and his research work anaerobic nitrogen fixing bacteria genus clostridium was very much acceptable and his suggestions was accepted published in newspaper Bangladesh observer and paper was published in book .in conference proceedings in first pages crop response correlations studies please see attached documents. The approach is multi-disciplinary, involving molecular biologics, plan breeders soil scientists and others.
4. It is happy to say. the un university has chosen to work on biological nitrogen fixation for several reasons. The research will be of immediate importance to developing countries. The results of this research are more readily available since it is carried out primarily in institutions rather than industrial laboratories. By catalyzing a greater interest in this field in developing countries the un universities hopes to create core groups of researchers which will then have the capacity to deal with other topics in the field of biotechnology.
5. A modest amount of the un university funding has been allocated to this project for research support, fellowship award and communication of results. Dr. Horta is very co –operative and willing to active a number of postgraduate or post-doctoral scientists from developing countries to work with him in his laboratories. Other advanced laboratories where scholars can participate in the research are invited to do the same. The visiting scholars should spend a period of six to twelve months in the host institutions. The host institutions are entitled to receive a compensation for their costs. More experienced scientists from developing countries may be supported t make scientific visits of one week to three months to other laboratories participating in the program. Consultants may also be hired for specific missions’ small sums of money may be used to strengthen research in certain individual discussed by the participants. As a participant we performed all task and implemented to the developing country still today there are lot of crop response.
6. Dr. Robert recognizing my expertise and experience in this field and dr. Robber kokke said to me he would like to invite med me in several workshop to discussed with him about the nitrogen fixing bacteria to participate in this and various workshops or to propose a qualified substitute if I Dr. Shelley can’t not attend but I attended in Netherlands, UK Finland etc. And that will be a highly technical worship comprising researchers working in this field it’s of the specific meetings objectives and provisional agendas are enclosed the major activity will be discussions on the states of current research as perceived by the individuals’ participants and ideas for further cooperative efforts.
7. I immediately response with my research works and proposal see on the first my name Dr. S. I. Shelley, Dr. Robert kokke would like to solicit my proposal on some aspects of the topic that my feel to be of immediate importance the enclosed projects proposal form outlines the information that should be included proposal should preferably be submitted in advance until 15 April 1984, they should be sent to dr. Robert kokke in Tokyo. Thereafter they should go to dr. I Waianae at irri, po box 933 manila Philippines and it was done by me dr. S. I. Shelley
8. Please see to attend the different meeting the una university will provide economy class round trip tickets from my academy institutes to los banos plus per diem for the days of the conference to be paid according to the un standard. And I confirmed and work out. Explained by Dr. Robert kokke.
10. After successfully projects completions sent me edited version of the recommendations of the Unu–irri workshops on nitrogen fixation in rhizosphere office held at irri, los banos, 28th April 3 May 1984
11. Dr. Robert kokke wishes also to take this opportunity to tanks me for participating in the workshop and for the valuable contribution which I made to it. And he hoped the workshop will be the beginning of fruitful co-operation of scientists in these areas
12. My suggestions and he mentioned in his letter Dr. Shelley wish me to revise or write my proposal to unu in the light of the discussions and the recommendations of the work and see please his appreciation letter and eventually I reedited and submitted to him. Please see enclosed book.
My recommendations were as follows: biological nitrogen fixations:
to have alternatives to intensive use of nitrogen fertilizers in the cultivation of rice., it is necessary to explore the potential of biological nitrogen fixation in rice fields. Among the known system, rhizosphere nitrogen fixation has been relatively underexploited. In view of this , the participants have agreed to recommend the establishments of a network of research in this areas under the unu program .after reviewing the current state of knowledge , it is feet that more fundamental research is needed before investing large amounts of money in field experiments the following aspects have been identified in which in depth studies are essential to have a clear picture of plant –bacteria associations:
1.survey of diverse rhizosphere ecosystems of rice for isolating and identifying
I. Active colonizers and
ii. Active nitrogen fixers.
Remarks my anaerobic bacteria considers active nitrogen fixers
2. Accurate and reproducible assessment of nitrogen fixation and colonization potential different isolates.
I made recommendation and we together took following declaration.
I. To have alternative to intensive use nitrogen fertilizers in the cultivation of rice, it is necessary to explore the potential of biological nitrogen fixation in rice fields. among the known systems, rhizosphere nitrogen fixation has been elatedly underexplored. In view of this, the participants have agreed to recommend the establishment of a network of research in this area under the unu program
ii. Note: after reviewing the current state of knowledge, it is felt that more fundamental research is needed before investing large amounts of money in field experiments. The following aspects have been identified in which in depth studies are essential to have clear picture of plant bacteria associations:
iii. A. Survey of diverse biosphere of rice for isolating ad identifying
iv. B. Active colonizers and ii active nitrogen fixers.
1. Accurate and reproducible assessment of nitrogen fixation and colonization potential of different isolates.
2. Accurate and reproducible assessment of the ability of rice cultivators to benefit from biological nitrogen fixation.
3. The meeting also recognized the necessity to increase the capability of young scientists from developing countries to apply molecular biological techniques to rhizosphere bnf through training in recognized centers,
in these areas the meeting has stressed the necessity of adopting the following uniform methodologies to arrive at meaningful comparisons of the results from different laboratories.
• isolations of strains
• the staring materials must be obtained from an actively fixing plant, as proven by acetylene reduction and must include roots as well as adhering soil.
• only very abundant bacteria are worth isolating as they are likely to be good colonizers.
• dilutions of the starting materials can be inoculated to diverse media, including sperm sphere models.
• among under investigated groups of nitrogen fixing bacteria are methane using bacteria , cellulolytic bacteria , hemicellulolytic bacteria and actinomycetes.
• a great effort must be made to determine isolates using proper available methodologies.
1. Assessment of strain potential
2. It seems important to distinguish the potential for nitrogen fixation and the potential for colonization.
3. The potential for nitrogen fixation can be assessed using monoaxenic systems such as the sperm sphere model.
4. Models closer to natural situations will be preferred if they show an at least similar comparable reproducibility.
5. Reference strain common to all participants shall be included in such comparative studies for Enterobacteriaceae it will be klebsiella oxytocic ng13 –nif +, nif _ pfr.
6. Hirotha and for azospirillum it will be a. Lipoferum 34h from phr.
8. Regarding the potential for colonization available methods counts, lectin rection, attachments measurements and microscopic observation must be evaluated and standardized before becoming routine methods.
• assessment of rice cultivator potential
• here again the sperm sphere model can be used as simple routine procedure as long as allowance is made for difference in the size seeds. Whenever possible results must be confirmed by experiments on older plants using 15 nitrogens’, this can be made by comparing cultivars to reference cultivars on the basis of their 15-nitrogen content either with uniformly labeled soil or using natural abundance of 15 nitrogen if a soil is available with a high value.
Reference rice proposed are: Oryza japonicat65, cycle duration 110 days, Oryza sativa ir58, cycle duration 100 days m, Oryza sativa ir42, cycle duration 120 days.
Detecting inactive lines seems as important as detecting very active ones. The former can become useful controls in further experiments.
In addition to rhizosphere biological nitrogen fixation, the meeting recognized the potential role of other process vesicular –arbuscular endomycorrhiza, growth promoting substances, other nitrogen fixing systems in relations to rice production which are beyond the scope of the proposed network.
If you have any questions concerning my petition, please contact me at the following address indicate or at tel. 1-(347)-285-4528.
Dr. Sherajul I. Shelley
Ph. D. MRSB.
1. WORK ADDRESS; HEAD OF MICROBIOLOGY LABORATORY.
MICOBIOLOGIST AND ASSISTANT QC MANAAGER, ACCUON LABS, BACTOLAB
PHARMACEUTICALS275. MARCUS BLVD. SUITE#N, HAUPPAUGE, NEW YORK 117
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